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Johnson/Case Lab Manual Supplement

by Christine L. Case, Skyline College

In this Lab Supplement, you will find a series of critical thinking questions intended to accompany Laboratory Experiments in Microbiology, 7th Edition, by Johnson/Case. These questions may also be used in conjunction with any laboratory manual that includes the experiments listed below. The questions are designed to help you interpret your lab results and to better understand microbial physiology. The photographs accompanying the questions are not intended to replace the experience of actually performing the experiments; rather, they are intended to supplement your lab experience and serve as points of reference.

If you are using the Johnson/Case manual, answers to Appendix B are found here.

To begin, click on the experiment on which you are working.

Experiment Exercise Number
(in Johnson/Case lab manual)
Gram Stain
Interpreting a Gram stain
#5
Acid Fast Stain
Interpreting an acid-fat stain
#6
Structural Stains
Capsule stain
Endospore stain
#7
Morphological Unknown
Constructing a dichotomous key
#8
Microbes in the Environment
Bacterial and fungal colonies
Nutrient broth
#9
Transfer of Bacteria: Aseptic Technique
Motility in a deep
Lactococcus
#10
Transfer of Bacteria: Aseptic Technique
Culture media
#10
Isolation of Bacteria by Dilution Techniques
Pour plates
Find the pure culture
Streptomyces
#11
Special Media for Isolating Bacteria
MSA
EMB
#12
Carbohydrate Catabolism
Starch hydrolysis
OF medium
#13
Fermentation of Carbohydrates
MR test
VP test
Fermentation tubes
#14
Protein Catabolism, Part 1
Urea hydrolysis
Gelatin hydrolysis
Litmus milk
#15
Protein Catabolism, Part 2
Indole test
Peptone iron agar
MIO
Phenylalanine deaminase
#16
Respiration
Oxidase test
Nitrate reduction
Catalase test
#17
Rapid Indentification Methods
Citrate
IMViC
Enterotube
API
#18
Oxygen and the Growth of Bacteria
Thioglycollate
#19
Determination of a Bacterial Growth Curve
Sample graphs
Critical Thinking Qu. #2
Cell numbers and Abs.
#20
Other Influences on Microbial Growth
Biofilms
Review quiz
In nutrient broth
#21
Physical Methods of Control: Heat
Graph of sample results
The Demonstration Plates
#22
Physical Methods of Control: Ultraviolet Radiation
UV radiation
#23
Chemical Methods of Control: Disinfectant and Antiseptics
Sample results
#24
Chemical Methods of Control: Antimicrobial Drugs
Antibiosis
Kirby-Bauer Test
#25
Effectiveness of Hand Scrubbing
Sample results
#25
Control of Gene Expression
Green Fluorescent Protein
Nitrate reductase
lac Operon
#27
Transformation of Bacteria
Isolation of DNA
Transformation
#29
DNA Fingerprinting
RFLPs
#30
Genetic Engineering
Recombinant DNA
#31
Ames Test for Detecting Possible Chemical Carcinogens
Ames Test
#32
Fungi: Yeasts
Yeast
#34
Fungi: Molds
Aspergillus
#35
Isolation of Bacteriophages
Bacteriophage
#38
Epidemiology
Serratia
Epidemiology
#40
Koch's Postulates
Koch's Postulates
Soft rot
Milky spore disease
#41
Blood Group Determination
Slide agglutination
#43
Agglutination Reactions
Sample results & Self-quiz
#44
ELISA Technique
Sample results
#44
Bacteria of the Skin
Coagulase test
Tellurite glycine agar
#46
Bacteria of the Mouth
MSB Agar
#47
Bacteria of the Gastrointestinal Tract
MacConkey Agar
TSI slant
PEA
#49
Bacteria of the Urogenital Tract
Pseudomonas
Neisseria Gonorrhoeae
#50
Microbes in Water: Multiple-Tube Techniques
MPN Test
MUG agar
#52
Microbes in Water: Membrabe Filter Techniques
EMB agar
#53
Microbes in Food: Contamination
Standard Plate Count
#54
Microbes Used in the Production of Foods
Beer
Chocolate
#54
Microbes in Soil: The Nitrogen and Sulfur Cycles
Winogradsky column
#56
Microbes in Soil: Bioremediation
Lipid hydrolysis
Enrichments
#57

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