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This activity contains 20 questions.
All of the following are types of light microscopy
Which of the following types of microscopy is most useful for viewing the internal structures of unstained specimens?
Which of the following types of microscopy is used to study living cells attached to surfaces, such as cancer cells and bacterial biofilms?
Which type of light microscopy is used to visualize stained specimens?
Which type of microscopy is used to identify pathogenic bacteria in clinical specimens?
Differential-interference contrast microscopy
Transmission electron microscopy
Resolution is great when using an electron microscope because the _____________ of the electron beam is much less than that of visible light.
All of the following are true for both TEM and SEM
Can be used to view specimens smaller than 0.2 micrometers.
The microscope is focused using electromagnetic lenses.
The illuminating source is an electron beam.
The specimen must be sectioned prior to viewing.
Black and white images are produced.
A three-dimensional image of a bacterium is achieved by using
transmission electron microscopy.
scanning electron microscopy.
Which type of microscope uses a metal and diamond probe that is gently forced down along the surface of a specimen?
Bacterial cells have an overall negative charge on their surface. If a bacterial smear is stained by a pink, acidic, water-soluble stain and then washed with water, which of the following describes the result?
Unstained cells with no background.
Unstained cells in a pink background.
Pink cells in a pink background.
Which of the following is not accomplished by fixing cells to a slide?
Cells are killed.
Cell structures such as the capsule are disturbed.
Cells are attached to the slide.
Cell structures are preserved.
Cell structures are differentiated.
A simple stain
differentiates one bacterial group from another.
requires multiple staining steps.
requires one stain and one decolorizing agent.
allows visualization of bacterial motility.
increases the contrast between the cell and the background.
In a negative staining procedure, the bacterial cells would be
brightly colored in a contrasting background.
the same color as the background and thus not easily observed.
unstained in a colored background.
stained in an unstained background.
attracted to the dye used in the staining procedure.
In the Gram stain, crystal violet is the
In the Gram stain, if the decolorizing step is deleted, gram-negative cells will appear ____at the completion of the staining procedure.
In the Gram stain, crystal violet remains in gram-positive cells after treatment with alcohol because crystal violet-iodine (CV-I) complexes are trapped
in thick layers of peptidoglycan.
in the outer membrane.
are distinguished from non-acid fast bacteria by the presence of
In the capsule stain using India ink, capsules are distinguished as _________surrounding cells.
In the Gram-stain procedure, a clear oval in the center of a cell could indicate:
lack of the central nucleoid.
the unstained bacterial chromosome.
presence of an endospore.
an unstained nucleus.
Which is common to the Gram stain and acid-fast stain?
Use of methylene blue as a counterstain.
Fixation of the smear prior to staining.
Use of acid alcohol for decolorizing.
Use of steam for stain penetration.
Use of iodine as a mordant.
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